gibson assembly master mix (New England Biolabs)
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gibson assembly master mix
Gibson Assembly Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gibson+assembly+master+mix/Gibson+Assembly+Master+Mix/pmc12967149-217-24-28
Average 99 stars, based on 6210 article reviews
Gibson Assembly Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6210 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gibson+assembly+master+mix/Gibson+Assembly+Master+Mix/pmc12967149-217-24-28
Average 99 stars, based on 6210 article reviews
gibson assembly master mix - by Bioz Stars,
2026-09
99/100 stars
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Polymerase Chain Reaction:Article Title: AI platform for CRISPR functional mapping and function-based drug design Article Snippet: The sgRNA oligos were amplified with PCR using Phusion High-Fidelity DNA Polymerase (NEB, M0530S). .. The PCR product was then gel purified using QIAquick Gel Extraction Kit (QIAGEN, 28704) and cloned using 10ng of PCR product and 25ng of digested pHKO9 for a 20μl reaction using a Article Title: Development of a tetracycline-inducible programmed ribosomal frameshifting platform for sensitive regulation of mammalian gene expression Article Snippet: .. The constructs were created using overhang extension polymerase chain reaction (PCR), followed by ligation with the Quick LigationTM Kit (NEB) or the Purification:Article Title: AI platform for CRISPR functional mapping and function-based drug design Article Snippet: The sgRNA oligos were amplified with PCR using Phusion High-Fidelity DNA Polymerase (NEB, M0530S). .. The PCR product was then gel purified using QIAquick Gel Extraction Kit (QIAGEN, 28704) and cloned using 10ng of PCR product and 25ng of digested pHKO9 for a 20μl reaction using a Article Title: Development of a suite of activatable plant synthetic promoter systems using a bacterial LysR-type transcriptional regulator FdeR Article Snippet: .. Briefly, DNA fragments were amplified from template plasmids or synthesized DNA fragments (listed in ) via the Q5 polymerase (New England BioLabs), purified with the Zymoclean gel DNA recovery kit (Zymo Research), and assembled with the Gel Extraction:Article Title: AI platform for CRISPR functional mapping and function-based drug design Article Snippet: The sgRNA oligos were amplified with PCR using Phusion High-Fidelity DNA Polymerase (NEB, M0530S). .. The PCR product was then gel purified using QIAquick Gel Extraction Kit (QIAGEN, 28704) and cloned using 10ng of PCR product and 25ng of digested pHKO9 for a 20μl reaction using a Clone Assay:Article Title: AI platform for CRISPR functional mapping and function-based drug design Article Snippet: The sgRNA oligos were amplified with PCR using Phusion High-Fidelity DNA Polymerase (NEB, M0530S). .. The PCR product was then gel purified using QIAquick Gel Extraction Kit (QIAGEN, 28704) and cloned using 10ng of PCR product and 25ng of digested pHKO9 for a 20μl reaction using a Transformation Assay:Article Title: Zebrafish Ace2N-mNeon expression toolkit for in vivo voltage imaging of neuronal synchrony and cardiac maturation Article Snippet: Inserts were generated by overlap-extension PCR using Phusion high-fidelity DNA polymerase (NEB), and promoter-containing backbones were amplified with KOD Xtreme Hot Start DNA Polymerase (Merck Sigma). .. Fragments were assembled with Plasmid Preparation:Article Title: The nuclear envelope protein TMEM209 is an integral component of the nuclear pore complex and interacts with Nup210 Article Snippet: .. For the Article Title: Development of a tetracycline-inducible programmed ribosomal frameshifting platform for sensitive regulation of mammalian gene expression Article Snippet: .. The constructs were created using overhang extension polymerase chain reaction (PCR), followed by ligation with the Quick LigationTM Kit (NEB) or the Construct:Article Title: Differential tolerance for SEA domain misfolding encodes a MAPK pathway-specific response Article Snippet: .. Plasmids were constructed by gap repair and Article Title: Development of a tetracycline-inducible programmed ribosomal frameshifting platform for sensitive regulation of mammalian gene expression Article Snippet: .. The constructs were created using overhang extension polymerase chain reaction (PCR), followed by ligation with the Quick LigationTM Kit (NEB) or the Amplification:Article Title: Development of a suite of activatable plant synthetic promoter systems using a bacterial LysR-type transcriptional regulator FdeR Article Snippet: .. Briefly, DNA fragments were amplified from template plasmids or synthesized DNA fragments (listed in ) via the Q5 polymerase (New England BioLabs), purified with the Zymoclean gel DNA recovery kit (Zymo Research), and assembled with the Synthesized:Article Title: Development of a suite of activatable plant synthetic promoter systems using a bacterial LysR-type transcriptional regulator FdeR Article Snippet: .. Briefly, DNA fragments were amplified from template plasmids or synthesized DNA fragments (listed in ) via the Q5 polymerase (New England BioLabs), purified with the Zymoclean gel DNA recovery kit (Zymo Research), and assembled with the Ligation:Article Title: Development of a tetracycline-inducible programmed ribosomal frameshifting platform for sensitive regulation of mammalian gene expression Article Snippet: .. The constructs were created using overhang extension polymerase chain reaction (PCR), followed by ligation with the Quick LigationTM Kit (NEB) or the |